An efficient method for isolation of RNA and DNA from plants containing polyphenolics
نویسندگان
چکیده
منابع مشابه
An efficient method for isolation of RNA and DNA from plants containing polyphenolics.
Isolation of high quality RNA and DNA from plants especially cotton (Gossypium hirsutum; family Malvaceae) is notoriously difficult. This problem has been attributed to high content of phenolic terpenoids and tannins present in cotton cells (Katterman, and Shattuck, 1983). These compounds bind to RNA and DNA upon cell lysis and cannot be removed by conventional extraction procedures. Such RNA i...
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The preparation of high quality DNA from polyphenoliccontaining plants such as field bean (Vicia faba), tomato (Lycopersicon esculentum), and potato (Solanum tuberositm) was difficult, because of DNA degradation mediated by secondary plant products such as phenolic terpenoids and tannins which may bind to DNA and/or RNA after cell lysis (John 1992). Common methods for DNA isolation from such pl...
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چکیده ندارد.
An Efficient Method For DNA Extraction From Paraffin Wax Embedded Tissues For PCR Amplification Of Human And Viral DNA
Background and Objective: Formalin-fixed paraffin-embedded tissues are a valuable source of DNA for molecular studies. We designed and optimized an efficient procedure for DNA extraction from formalin-fixed paraffin embedded tissues. Materials and Methods: Seventy three blocks of cervical paraffin-embedded tissues were investigated. DNA was extracted using 45 minutes boiling in alkaline sol...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1992
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/20.9.2381